Plasmid
Part:BBa_K3963000:Design
Designed by: Silja Malkewitz Group: iGEM21_Heidelberg (2021-09-30)
pBWB162
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1364
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A suitable origin of replication for natural transformation in Acinetobacter baylyi ADP1 was required. A BioBrick prefix was deleted from the original sequence for compatibility of RFC 10, 21, 23, 25.
Source
pBWB162 can be bought from Addgene https://www.addgene.org/140634/