Plasmid

Part:BBa_K3963000:Design

Designed by: Silja Malkewitz   Group: iGEM21_Heidelberg   (2021-09-30)


pBWB162


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1364
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A suitable origin of replication for natural transformation in Acinetobacter baylyi ADP1 was required. A BioBrick prefix was deleted from the original sequence for compatibility of RFC 10, 21, 23, 25.

Source

pBWB162 can be bought from Addgene https://www.addgene.org/140634/


References